Incorporation of C14-labeled Acetate into Lipids by Macrophages in Vitro.
نویسندگان
چکیده
منابع مشابه
Lipid Transformations in Plastids of Bean Leaves and Pepper Fruits.
A few reports have appeared concerning the synthesis of plastid lipids. Stumpf and James (17) and Stumpf et al. (18) found that both saturated and unsaturated fatty acids may be formed from acetate by chloroplast preparations. However, in some bluegreen algae, Bloch et al. (4) found that long-chain monounsaturates are synthesized by oxidative desaturation of corresponding saturates. Kates (9) s...
متن کاملIncorporation of (2-14C)acetate into lipids of mink (Mustela vison) liver and intestine during in vitro and in vivo treatment with aflatoxin B1.
The in vitro and in vivo incorporation of (2-14C)acetate into lipids of mink (Mustela vison) liver and intestines was studied. In vitro, a dose of aflatoxin B1 as small as 7.5 mug/ml of medium reduced by 20% the amount of (2-14C)acetate incorporated into lipids of mink liver slices, whereas 180 mug caused 76% reduction in the synthesis of lipids from the radioactive precusor. Similar inhibition...
متن کاملMetabolism of adipose tissue : incorporation of isoleucine carbon into lipids by slices of adipose tissue
Slices from adipose tissue and liver were incubated with uniformly labeled C14-isoleucine and the respiratory carbon dioxide, fatty acids, and nonsaponifiable lipids were isolated and analyzed for radioactivity. Organic acids were qualitatively identified by chromatography and autoradiography. Adipose tissue oxidized isoleucine to carbon dioxide a t a rate greater than that of liver. Conversion...
متن کاملThe synthesis of radioactive cholesterol and fatty acids in vitro.
It has been established that rat liver slices are able to convert acetate (l), acetone (2), pyruvate (3, 4), butyrate, hexanoate, and octanoate (4) to cholesterol and long chain fatty acids. Zabin and Bloch (5) and Price and Rittenberg (6) administered 1 ,3-C14-acetone to rats and demonstrated that it is converted to fatty acids and cholesterol. The former investigators (5) also reported that m...
متن کاملIncorporation of Radiolabelled Substrates into Mycobacteri- al Lipids of M. Bovis BCG and M. Kansasii
Background: Diseases caused by microorganisms of the genus Mycobacterium continue to be a serious problem. The cell envelope constitutes up to 60% of the dry weight of lipids. The cell wall of the mycobacterium is essential for their growth and survival in the infected host. The cell wall lipids have been isolated from M. bovis BCG and M. kansasii mycobacterial strains and studied the incorpora...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of lipid research
دوره 5 شماره
صفحات -
تاریخ انتشار 1964